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fe2 detection probeferroorange  (Dojindo Labs)
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Dojindo Labs fe2 detection probeferroorange
Fig. 5. C5b-9 induced HK-2 cells ferroptosis. Complement C5b6 and normal human serum, the source of complement C7, C8 and C9, were used to assemble C5b-9 on the cytomembrane of HK-2 cells. (A) Cell viability of HK-2 cells after 24 hr C5b-9 assembly. (B) ACSL4, COX2 and GPX4 of HK-2 cells were detected using immunoblotting. (C-E) Acsl4, Ptgs2 and Gpx4 mRNAs level of HK-2 cells were measured using RT-PCR. (F) GSH level of HK-2 cells. (G) MDA level of HK-2 cells. (H) Lipid ROS was detected by BODIPY™ 581/591 C11. The green fluorescence represents oxidized lipid and the red fluorescence represents non-oxidized lipid. The scale bar represents 50 µm. (I) <t>Fe2+</t> was detected by FerroOrange dye. The scale bar represents 50 µm. Data are shown as mean ± SEM. (n = 4–6 per group). * P < 0.05.
Fe2 Detection Probeferroorange, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5. C5b-9 induced HK-2 cells ferroptosis. Complement C5b6 and normal human serum, the source of complement C7, C8 and C9, were used to assemble C5b-9 on the cytomembrane of HK-2 cells. (A) Cell viability of HK-2 cells after 24 hr C5b-9 assembly. (B) ACSL4, COX2 and GPX4 of HK-2 cells were detected using immunoblotting. (C-E) Acsl4, Ptgs2 and Gpx4 mRNAs level of HK-2 cells were measured using RT-PCR. (F) GSH level of HK-2 cells. (G) MDA level of HK-2 cells. (H) Lipid ROS was detected by BODIPY™ 581/591 C11. The green fluorescence represents oxidized lipid and the red fluorescence represents non-oxidized lipid. The scale bar represents 50 µm. (I) Fe2+ was detected by FerroOrange dye. The scale bar represents 50 µm. Data are shown as mean ± SEM. (n = 4–6 per group). * P < 0.05.

Journal: Ecotoxicology and environmental safety

Article Title: C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice.

doi: 10.1016/j.ecoenv.2022.114020

Figure Lengend Snippet: Fig. 5. C5b-9 induced HK-2 cells ferroptosis. Complement C5b6 and normal human serum, the source of complement C7, C8 and C9, were used to assemble C5b-9 on the cytomembrane of HK-2 cells. (A) Cell viability of HK-2 cells after 24 hr C5b-9 assembly. (B) ACSL4, COX2 and GPX4 of HK-2 cells were detected using immunoblotting. (C-E) Acsl4, Ptgs2 and Gpx4 mRNAs level of HK-2 cells were measured using RT-PCR. (F) GSH level of HK-2 cells. (G) MDA level of HK-2 cells. (H) Lipid ROS was detected by BODIPY™ 581/591 C11. The green fluorescence represents oxidized lipid and the red fluorescence represents non-oxidized lipid. The scale bar represents 50 µm. (I) Fe2+ was detected by FerroOrange dye. The scale bar represents 50 µm. Data are shown as mean ± SEM. (n = 4–6 per group). * P < 0.05.

Article Snippet: Fe2+ detection probeFerroOrange (Cat. F374) was from DOJINDO.

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Fluorescence

Fig. 6. Reducing Cytosolic Ca2+ Level Alleviated C5b-9-Induced Ferroptosis. (A) Relative cytosolic Ca2+ concentration of TCE-sensitized mouse primary TECs was detected by monitoring the fluorescence intensity at Ex/Em = 490/525 nm using multimode plate reader (PerkinElmer). (B) Relative cytosolic Ca2+ concentration of HK-2 cells was detected. (C) Cell viability of HK-2 cells after C5b-9 and BAPTA-AM co-treated. (D-F) Acsl4, Ptgs2 and Gpx4 mRNAs level of HK-2 cells were measured using RT-PCR. (G) GSH level of HK-2 cells. (H) MDA level of HK-2 cells. (I) Lipid ROS was detected by BODIPY™ 581/591 C11. The green fluorescence represents oxidized lipid and the red fluorescence represents non-oxidized lipid. The scale bar represents 50 µm. (J) Fe2+ was detected by FerroOrange dye. The scale bar represents 50 µm. Data are shown as mean ± SEM. (n = 4–6 per group). *P < 0.05.

Journal: Ecotoxicology and environmental safety

Article Title: C5b-9 mediates ferroptosis of tubular epithelial cells in trichloroethylene-sensitization mice.

doi: 10.1016/j.ecoenv.2022.114020

Figure Lengend Snippet: Fig. 6. Reducing Cytosolic Ca2+ Level Alleviated C5b-9-Induced Ferroptosis. (A) Relative cytosolic Ca2+ concentration of TCE-sensitized mouse primary TECs was detected by monitoring the fluorescence intensity at Ex/Em = 490/525 nm using multimode plate reader (PerkinElmer). (B) Relative cytosolic Ca2+ concentration of HK-2 cells was detected. (C) Cell viability of HK-2 cells after C5b-9 and BAPTA-AM co-treated. (D-F) Acsl4, Ptgs2 and Gpx4 mRNAs level of HK-2 cells were measured using RT-PCR. (G) GSH level of HK-2 cells. (H) MDA level of HK-2 cells. (I) Lipid ROS was detected by BODIPY™ 581/591 C11. The green fluorescence represents oxidized lipid and the red fluorescence represents non-oxidized lipid. The scale bar represents 50 µm. (J) Fe2+ was detected by FerroOrange dye. The scale bar represents 50 µm. Data are shown as mean ± SEM. (n = 4–6 per group). *P < 0.05.

Article Snippet: Fe2+ detection probeFerroOrange (Cat. F374) was from DOJINDO.

Techniques: Concentration Assay, Fluorescence, Reverse Transcription Polymerase Chain Reaction